PDF] NO2- and NO3- enhance cold atmospheric plasma induced cancer cell death by generation of ONOO

By adding nontoxic concentration of additional nitrite and nitrate in the medium, this study found that anti-tumor effect of CAP treatment was enhanced in the same discharging parameters and proposed that more nitrogen supplies such as nitriteand nitrate could increase the production of RNS especially ONOO- and resulted in a better killing effect to cancer cells. Cold atmospheric plasma (CAP) is a rapidly developed technology that has been widely applied in biomedicine especially in cancer treatment. Due to the generation of various active species in plasma, CAP could induce various tumor cells death and showed a promising potential in cancer therapy. To enhance the biological effects of gas plasma, changing the discharging parameters is the most commonly used method, yet increasing discharging power will lead to a higher possibility of simultaneously damage surrounding tissues. In this study, by adding nontoxic concentration of additional nitrite and nitrate in the medium, we found that anti-tumor effect of CAP treatment was enhanced in the same discharging parameters. By microplate reader and cell flow cytometer we measured several extracellular and intracellular RONS and found that ONOO- was mostly correlated with the enhanced cancer cell killing effect. We proposed that more nitrogen supplies such as nitrite and nitrate could increase the production of RNS especially ONOO- and resulted in a better killing effect to cancer cells. Our results provided a new strategy to enhance the antitumor effect by plasma jet treatment without changing the discharging parameters.Cold atmospheric plasma (CAP) is a rapidly developed technology that has been widely applied in biomedicine especially in cancer treatment. Due to the generation of various active species in plasma, CAP could induce various tumor cells death and showed a promising potential in cancer therapy. To enhance the biological effects of gas plasma, changing the discharging parameters is the most commonly used method, yet increasing discharging power will lead to a higher possibility of simultaneously damage surrounding tissues. In this study, by adding nontoxic concentration of additional nitrite and nitrate in the medium, we found that anti-tumor effect of CAP treatment was enhanced in the same discharging parameters. By microplate reader and cell flow cytometer we measured several extracellular and intracellular RONS and found that ONOO- was mostly correlated with the enhanced cancer cell killing effect. We proposed that more nitrogen supplies such as nitrite and nitrate could increase the production of RNS esp

PDF) Combination of metformin and cold atmospheric plasma induces glioma cell death to associate with c-Fos

PDF] NO2- and NO3- enhance cold atmospheric plasma induced cancer cell death by generation of ONOO

The synergistic effect between hydrogen peroxide and nitrite, two long-lived molecular species from cold atmospheric plasma, triggers tumor cells to induce their own cell death - ScienceDirect

PDF) Cold Atmospheric Plasma: A Promising Controller of Cancer Cell States

The synergistic effect between hydrogen peroxide and nitrite, two long-lived molecular species from cold atmospheric plasma, triggers tumor cells to induce their own cell death. - Abstract - Europe PMC

Important parameters in plasma jets for the production of RONS in liquids for plasma medicine: A brief review

Enhanced Generation of Reactive Species by Cold Plasma in Gelatin Solutions for Selective Cancer Cell Death

IJMS, Free Full-Text

Cold Atmospheric Plasma and Plasma-Activated Medium Trigger RONS-Based Tumor Cell Apoptosis

Frontiers Cold Atmospheric Plasma Ameliorates Skin Diseases Involving Reactive Oxygen/Nitrogen Species-Mediated Functions

Frontiers Cold Atmospheric Plasma Ameliorates Skin Diseases Involving Reactive Oxygen/Nitrogen Species-Mediated Functions

Dynamics of Singlet Oxygen-Triggered, RONS-Based Apoptosis Induction after Treatment of Tumor Cells with Cold Atmospheric Plasma or Plasma-Activated Medium

Biomedicines, Free Full-Text